Figure 1.

Description of the establishment of crypt-derived 3D avian intestinal organoids. (A) Schematic diagram showing avian intestinal crypts isolated from a broiler chicken. The avian intestines were collected, cut open longitudinally, washed 3 times in cold PBS, cut into small pieces, and incubated in EDTA to release the crypts. The crypts were purified through differential centrifugation and filtration with 100 µm and 70 µm filters. Purified crypts were embedded in Matrigel surrounded in organoid growth media. (B) Representative ceca-cross section stained with hematoxylin-eosin (H&E). Black arrows indicate examples of crypts. Scale bar = 100 µm. (C) Representative image of ceca cross-section stained with H&E after the removal of most crypts following EDTA chelation. Scale bar = 100 µm. (D) Purified crypts after differential centrifugation and filtration.