Figure 3.

Morphology of avian intestinal organoids.

A representative bright-field image of a cecal organoid derived from 20-wk-old layers (A), a cecal organoid of 4-day-old broilers (B), and small intestines (including duodenum, jejunum, and ileum) of 19-day-old chick embryos (C), and orgnoids derived from ileum of day-14-old broilers after one mo cryopreservation (D) are shown. Image (A) and (B) were captured with an Olympus FV 1000 confocal microscope, image (C) was recorded with an all-in-one BZ-X800 Keyence Fluorescence Microscope, and image (D) was captured with a Nikon Eclipse Ti microscope. All organoids were cultured in the media for 5 d. Scale bar = 100 µm.