Figure 5.

Western blot analysis of different cell types from avian cecal organoids using protein biomarkers.

Cecal organoids in a Matrigel were collected with TrypLE Express and washed with ice-cold PBS. Total protein from organoids was extracted with radioimmunoprecipitation assay buffer (RIPA). Fifty micrograms of protein from cecal organoids were resolved in the 4 to 12% SDS-PAGE gradient gels. Immunoblotting of whole organoid protein extracts showed the presence of Lgr5 (a stem cell marker), E-cadherin (an epithelial cell marker), lysozyme (a Paneth cell marker), and chromogranin A (an enteroendocrine cell marker).