Table 1.
Target gene | Primer Sequence (5’ to 3’) | Amplicons (bp) | Reference | |
---|---|---|---|---|
LGR5 | For | CCTTTATCAGCCCAGAAGTGA | 338 | Li et al., 2018 |
Rev | TGGAACAAATGCTACGGATG | |||
E-cadherin | For | ACTGGTGACATTATTACCGTAGCA | 226 | Tiwari et al., 2013 |
Rev | TAGCCACTATGACATCCACTCTGT | |||
Lysozyme | For | GACGATGTGAGCTGGCAG | 225 | Wang et al., 2016 |
Rev | GGATGTTGCACAGGTTCC | |||
Chromogranin A | For | TGAATAAAGGGGACACTAAGG | 337 | Dr. Berghman |
Rev | AGCTCAGCCAGGGATG | |||
Muc2 | For | CAGCACCAACTTCTCAGTTCC | 102 | Zhang et al., 2015 |
Rev | TCTGCAGCCACACATTCTTT | |||
Olfm4 | For | GACTGGCTCTCTGGATGACC | 108 | Li et al., 2018 |
Rev | AGCGTTGTGGCTATCACTTG | |||
Alpha-SMA | For | AGGACAGCACTGCCCTTGTTT | 136 | Tavares et al., 2018 |
Rev | CCCATACCAACCATCACACCCT | |||
Vimentin | For | GAAGCTGCTAACTACCAGGACACT | 184 | Tiwari et al., 2013 |
Rev | TAGGCATGTTAATCCTGCTCTCTT | |||
GAPDH | For | AGAACATCATCCCAGCGT | 182 | Shang et al., 2014 |
Rev | AGCCTTCACTACCCTCTTG | |||
Beta-Actin | For | TGCTGTGTTCCCATCTATCG | 150 | Bai al., 2018 |
Rev | TTGGTGACAATACCGTGTTCA |