REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, peptides, and recombinant proteins
PolyJet	SignaGen Laboratories	Cat# SL100688
Poly-D-Lysine	Sigma-Aldrich	Cat# P6407
Boric acid	Sigma-Aldrich	Cat# 10043-35-3
Sodium tetraborate	Sigma-Aldrich	Cat# 1330-43-4
HEPES	Sigma-Aldrich	Cat# H3375
Forskolin (Fsk)	Calbiochem	Cat# 344281
3-isobutyl-1-methylxanthine (IBMX)	Sigma-Aldrich	Cat# I7018
35-mm glass-bottom imaging dish	Cellvis	Cat# D35-20-1.5-N
96 well glass bottom plate	Cellvis	Cat# P96-1.5H-N
Experimental models: Cell lines
HEK293T	ATCC	ATTC Cat# CRL-11268
Recombinant DNA
pcDNA3.1(+)-ExRai-AKAR2	Zhang et al. (2021a)	Addgene Plasmid #161753
pcDNA3.1(+)-ExRai-AKAR2 (T/A)	Zhang et al. (2021a)	Addgene Plasmid #161754
Software and algorithms
PRISM	GraphPad	https://www.graphpad.com/scientific-software/prism/
ImageJ	National Institutes of Health	https://imagej.nih.gov/ij/download.html
METAFLUOR 7.7	Molecular Devices	https://www.moleculardevices.com/products/cellular-imaging-systems/acquisition-and-analysis-software/metamorph-microscopy#Resources
Spark Control	TECAN	https://lifesciences.tecan.com/plate-reader-live-cell-imaging-cytometry?p=tab--4
Excel	Microsoft	https://www.microsoft.com/en-us/microsoft-365/excel
Other
Zeiss AxioObserver Z1 microscope	Carl Zeiss	https://www.zeiss.com/microscopy/us/products/light-microscopes/axio-observer-for-biology.html#features
Lambda 10-2 Filter-changer	Sutter Instruments	LB10-B/IQ
Fluorescence microplate reader	TECAN	SPARK 20M
Evolve 512 EMCCD Camera	TELEDYNE PHOTOMETRICS	Evolve 512 EMCCD Camera