Table 2.
Primer specific sequences used for RT-qPCR analysis.
| Genes | Primer sequence (5′-3′) | Amplicon size (bp) | Genbank identification |
|---|---|---|---|
| GAMT | F: CGTGAAGGGCAAATACAGCG | 146 | XM_040692773.1 |
| R: GGAAGGAGTAGTAGCGGCAC | |||
| CreaT | F: TGAACTACAAACCGCTGACG | 120 | JN628439.2 |
| R: GCTCGTAGATAACGGTGCAG | |||
| LKB1 | F: TGAGAGGGATGCTTGAATACGA | 138 | NM_001045833.1 |
| R: ACTTGTCCTTTGTTTCTGGGC | |||
| MO25α | F: CGTGTTTAAGGTGTTTGTAGCC | 245 | XM_015277058.3 |
| R: AGCAACTGCTGAATTTGGGT | |||
| STRADα | F: TAAACCCGAACGGATTAGGCG | 182 | NM_001305191.1 |
| R: TGCTGTCTGGGAGGAAGTTG | |||
| AMPKα1 | F: ATCTGTCTCGCCCTCATCCT | 125 | NM_001039603.1 |
| R: CCACTTCGCTCTTCTTACACCTT | |||
| AMPKα2 | F: GGGACCTGAAACCAGAGAACG | 215 | NM_001039605.1 |
| R: ACAGAGGAGGGCATAGAGGATG | |||
| β-actin | F: ATCCGGACCCTCCATTGTC | 120 | NM_205518.1 |
| R: AGCCATGCCAATCTCGTCTT |
Abbreviations: AMPKα1, adenosine 5′-monophosphate-activated protein kinase α1; AMPKα2, adenosine 5′-monophosphate-activated protein kinase α2; CreaT, creatine transporter; GAMT, S-adenosyl-L-methionine: guanidinoacetate N-methyltransferase; LKB1, liver kinase B1; MO25α, mouse protein 25α; STRADα, STE20-related adaptor α.