. 2021 Dec 7;101(3):101653. doi: 10.1016/j.psj.2021.101653

Table 6.

Effects of dietary graded creatine nitrate (CrN) supplementation on glycolytic parameters and activities of glycolytic enzymes in Pectoralis major muscle of broilers.

Items	Treatments¹					SEM	P value
Items	Control	GAA₆₀₀	CrN₃₀₀	CrN₆₀₀	CrN₉₀₀	SEM	ANOVA	Linear²	Quadratic²
Glycolytic parameters
Glycogen (μmol/g)	5.26^b	5.54^b	5.15^b	6.34^a	6.53^a	0.14	0.001	<0.001	0.513
Lactic acid (μmol/g)	131.42^a	126.34^ab	130.79^a	125.43^b	125.56^b	0.85	0.042	0.010	0.844
GP (μmol/g)	141.94	137.42	141.08	138.11	138.62	0.81	0.321	0.132	0.717
Activities of glycolytic enzymes
HK (U/mg of protein)	14.44	12.34	13.88	12.51	11.67	0.69	0.722	0.208	0.936
PFK (U/mg of protein)	56.93^a	53.59^a	52.80^a	47.61^b	45.66^b	0.92	<0.001	<0.001	0.480
PK (U/g of protein)	51.76^a	38.92^b	36.18^b	34.99^b	38.93^b	1.48	0.001	0.002	0.001
LDH (U/mg of protein)	4.30^a	3.61^ab	3.08^b	3.15^b	2.97^b	0.13	0.003	0.001	0.044

Abbreviations: GP, glycolytic potential (GP = 2 × [glycogen] + [lactic acid]); HK, hexokinase; LDH, lactate dehydrogenase; PFK, phosphofructokinase; PK, pyruvate kinase.

^a,b

Different letters in the mean value of the same row indicate a significant difference (P < 0.05). The data are represented as the mean value and pooled SEM (n = 6).

Control, basal diet; GAA₆₀₀, basal diet supplemented with 600 mg/kg guanidinoacetic acid (GAA); CrN₃₀₀, CrN₆₀₀, and CrN₉₀₀, basal diets supplemented with 300, 600, and 900 mg/kg CrN, respectively.

Orthogonal polynomials were used to estimate the linear and quadratic effects of dietary CrN supplementation among the control, CrN₃₀₀, CrN₆₀₀, and CrN₉₀₀ groups.