. 2021 Dec 9;101(3):101635. doi: 10.1016/j.psj.2021.101635

Table 2.

Effect of lecithin and vitamin E on sperm characteristics of Hubbard M99 Line A grandparent roosters.

		Total motility (%)	Progressive motility (%)	VSL (μm/s)	VCL (μm/s)	VAP (μm/s)	STR (%)	LIN (%)	ALH (μm)
Treatments¹
Lecithin, %	Vitamin E, ppm
0	0	86.04^b	40.82^ab	73.35	181.61	42.65	54.89	40.16	7.70
0	300	86.79^b	37.83^b	74.80	184.48	42.19	52.60	40.32	8.23
1	0	86.20^b	38.44^b	74.22	184.81	44.14	53.88	39.84	7.83
1	300	90.46^a	43.91^a	74.44	181.37	40.68	54.49	40.32	8.17
2	0	85.31^b	39.07^b	73.26	181.03	42.82	54.58	40.00	7.95
2	300	90.10^a	43.40^a	73.67	184.54	44.22	55.29	39.52	8.32
SEM Pooled		1.476	1.758	2.478	2.898	1.775	1.447	1.607	0.509
Main effects
Lecithin, %
0		86.42^b	39.33	74.08	183.05	42.42	53.75	40.32	7.97
1		88.33^a	41.18	74.33	183.08	42.41	54.19	40.16	8.00
2		87.71^ab	41.24	73.47	182.77	43.52	54.94	39.84	8.14
Vitamin E, ppm
0		85.85^b	39.44^b	73.61	182.48	43.20	54.45	40.00	7.83^b
300		89.12^a	41.71^a	74.30	183.45	42.36	54.13	40.00	8.24^a
P-value
Lecithin		0.047	0.067	0.698	0.984	0.359	0.266	0.811	0.746
Time		0.209	0.234	0.140	<0.0001	0.001	0.0004	0.0001	<0.0001
Vitamin E		<0.0001	0.004	0.426	0.529	0.255	0.593	0.949	0.041
Lecithin × Time		0.035	0.071	0.190	0.368	0.185	0.048	0.392	0.364
Lecithin × Vitamin E		0.022	<0.0001	0.829	0.141	0.073	0.083	0.805	0.906
Vitamin E × Time		0.011	0.001	0.058	0.466	0.094	0.586	0.051	0.241
Lecithin × Vitamin E × Time		0.440	0.054	0.251	0.027	0.112	0.345	0.294	0.258

Data are presented as means ± SEM (n = 6 roosters/each treatment).

Superscripts with different means within column differ significantly at P ≤ 0.05.

Roosters received their diets at 45 wk of age for 60 d based on experimental design as follows: 1) basal diet, 2) basal diet supplemented with 300 mg/kg vitamin E, 3) basal diet supplemented with 1% lecithin, 4) basal diet supplemented with 1% lecithin along with 300 mg/kg vitamin E, 5) basal diet supplemented with 2% lecithin, and 6) basal diet supplemented with 2% lecithin along with 300 mg/kg vitamin E.

Abbreviations: ALH, amplitude of lateral head displacement; LIN, linearity; STR, straightness; VAP, average path velocity; VCL, curvilinear velocity; VSL, straight-line velocity.