Table 4.

Effect of lecithin and vitamin E on DNA fragmentation, membrane integrity, morphology, viability, and malondialdehyde (MDA).

		DNA fragmentation (%)	Membrane integrity (%)	Morphology (%)	Viability (%)	MDA (nmol/mL)
Treatments1
Lecithin, %	Vitamin E, ppm
0	0	6.35	82.86b	12.53	86.50b	2.52a
0	300	6.48	82.33b	12.45	86.59b	2.22b
1	0	6.84	83.49b	12.31	87.68b	2.59a
1	300	5.54	88.13a	11.82	92.64a	1.98c
2	0	6.46	84.10b	11.59	87.52b	2.55a
2	300	6.72	85.93ab	11.55	88.92b	2.01bc
SEM Pooled		1.089	3.056	1.110	1.649	0.149
Main effects
Lecithin, %
0		6.41	82.60b	12.49	86.55b	2.37
1		6.19	85.81a	12.07	90.31a	2.30
2		6.59	85.02a	11.57	88.23b	2.28
Vitamin E, ppm
0		6.55	83.48b	12.14	87.24b	2.55a
300		6.25	85.46a	11.94	89.53a	2.07b
P value
Lecithin		0.758	0.003	0.235	0.0004	0.142
Time		0.597	0.002	0.841	<0.0001	<0.0001
Vitamin E		0.500	0.009	0.642	0.002	<0.0001
Lecithin × Time		0.223	0.003	0.230	0.0003	0.156
Lecithin × Vitamin E		0.296	0.023	0.886	0.009	0.014
Vitamin E × Time		0.148	0.278	0.514	0.0009	<0.0001
Lecithin × Vitamin E × Time		0.462	0.427	0.347	0.308	0.024

Data are presented as means ± SEM (n = 6 roosters/each treatment).

Superscripts with different means within column differ significantly at P ≤ 0.05.

¹Roosters received their diets at 45 wk of age for 60 d based on experimental design as follows: 1) basal diet, 2) basal diet supplemented with 300 mg/kg vitamin E, 3) basal diet supplemented with 1% lecithin, 4) basal diet supplemented with 1% lecithin along with 300 mg/kg vitamin E, 5) basal diet supplemented with 2% lecithin, and 6) basal diet supplemented with 2% lecithin along with 300 mg/kg vitamin E.

Sperm Parameters: DNA Fragmentation (Acridine Orange test), plasma membrane integrity (hypo osmotic swelling test), morphology (Hancock solution), viability (Annexin V/Propidium iodide), and lipid peroxidation index (Malondialdehyde) were assessed.