FIG 2.
Subcloning of odilorhabdin BGC locus and putative ODL resistance genes. Odilorhabdin self-resistance gene identification was done by subcloning of the odl-BGC locus of X. nematophila. The odl-BGC locus of X. nematophila cloned into XL1 Blue MRF′ confers resistance to odilorhabdin. In order to identify the odilorhabdin self-resistance gene, subcloning of the three upstream genes and the seven downstream genes from the odl-BGC locus was realized by PCR amplification using appropriate primers into pUC19 vector under the control of the Plac promoter. On the right are MICs of NOSO-95C for XL1 Blue MRF′ carrying the entire cloned fragment after induction with aTc (200 ng/mL) or subcloned genes after induction with IPTG (0.2 mM). pACYC184 and pUC19 were used as controls.