FIG 3.

Characterization of acetylated NOSO-95C. (A) Antibacterial activity against E. coli ATCC 25922 of NOSO-95C after various reaction times (15 min, 4 h, or 24 h) with acetyl-CoA (2), odilorhabdin acetyltransferase (OatA) (3), or both (4). OatA with acetyl-CoA was used as a negative control (1). (B) Inhibition of synthesis of the GFP reporter protein in the E. coli cell-free transcription-translation system by NOSO-95C after various reaction times (15 min, 4 h, or 24 h) with acetyl-CoA, OatA, or both. OatA with acetyl-CoA was used as a negative control. Gentamicin (10 μM) was used as a positive control. The data shown are the means of three experiments ± SD.