TABLE 3.
MICs of NOSO-95C against E. coli recombinant strains expressing oatA or oatA-like genes and X. nematophila wild-type or mutant strainsa
Type of strain | MIC (μg/mL) of NOSO-95C |
---|---|
E. coli expressing oatA from Photorhabdus | |
XL1(Plac-oatA-Pla-HP88) | 512 |
XL1(Plac-oatA-Pla-TT01) | 512 |
XL1(Plac-oatA-Pk) | 512 |
XL1(Plac-oatA-Plu) | 1,024 |
E. coli expressing oatA-like from Xenorhabdus | |
XL1(Plac-oatA-like-Xc-JM26) | 8 |
XL1(Plac-oatA-like-Xc-DSM 17905) | 8 |
XL1(Plac-oatA-like-Xh) | 32 |
XL1(Plac-oatA-like-Xi) | 4 |
XL1(Plac-oatA-like-Xs) | 8 |
Controls | |
XL1 (pUC19) | 8 |
XL1(Plac-oatA-Xn) | 1,024 |
X. nematophila strain F1 | |
Wild type | >2,048 |
Mutant strain (ΔngrA) | >2,048 |
Double mutant strain (ΔngrA ΔoatA) | 256 |
Pla, P. laumondii; Pk, P. kayaii KR04; Plu, P. luminescens Hb; Xc, X. cabanillasii; Xh, X. hominickii DSM 17903; Xi, X. innexi UY61; Xs, X. stockiae DSM 17904.