TABLE 2.
Primers used in this worka
| Primer name | Primer sequence (5′→3′) |
|---|---|
| cobS pCV1 F | NNGCTCTTCNTTCATGAGTAAGCTGTTTTGGGC |
| cobS pCV1 R | NNGCTCTTCNTTATAACAGAGCCAGCAGAA |
| CobS NdeI F | NNNNCATATGATGAGTAAGCTGTTTTGG |
| CobS KpnI R | NNNNGGTACCTCATAACAGAGCCAGCAG |
| CobC EcoRI F | NNNGAATTCGAGGAATACCATGCGA |
| CobC HindIII R | NNNAAGCTTTCACTCAGGCCGCCA |
| CobC NheI F | NNNGCTAGCGAGGAATACCATGCGA |
| CobC SacI R | NNNGAGCTCTCACTCAGGCCGCCA |
| PspA EcoRI | NNNNGAATTCATGGGTATTTTTTCTCGC |
| PspA HindIII | NNNNAAGCTTTTATTGATTGTCTTGCTT |
a
All primers used in this work were synthesized by Integrated DNA Technologies (IDT, Coralville, IA).