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. 2022 Jan 11;13(1):e02785-21. doi: 10.1128/mbio.02785-21

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Copyright © 2022 Lin et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 4 — The host protection effect caused by ZNF2 overexpression requires capsule. (A) Images of cells of wild-type H99, LW10 (ZNF2^oe), NE644 (cap59Δuge1Δ), and KH35 (ZNF2^oecap59Δuge1Δ) when cultured in YPD medium (1st column) and RPMI medium (2nd column with Indian ink staining); and colony immunoblot of these colonies probed with HK-ZNF2^oe-vaccinated serum. (B) The relative transcript level of ZNF2 in wild-type H99, NE644 (cap59Δuge1Δ) and KH35 (ZNF2^oecap59Δuge1Δ) after overnight culture in YPD. The transcript level of TEF1 of each sample was used as the internal control. (C-D) Two independent animal experiments testing the vaccination effect of heat-killed ZNF2^oe cells in the wild-type background or in the acapsular mutant background using two slightly different vaccination regimens. The acapsular mutant cap59Δuge1Δ was included as a control in both experiments. The no vaccination group was included in (C) and the group vaccinated with heat-killed H99 cells was included in (D) as additional controls.