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. 2021 Nov 29;14(1):e14678. doi: 10.15252/emmm.202114678

Figure 2. Suppression of O‐GlcNAcylation downregulates pro‐inflammatory cytokine/chemokine production induced by Stx2a intoxication.

Figure 2

  1. Timeline for establishment of differentiated macrophage‐like THP‐1 cells (D‐THP‐1) from monocytic THP‐1 cells.
  2. ELISAs were used to analyze the inhibitory effect of OSMI‐1 (10 µM, final) on cytokine and chemokine production from THP‐1 cells exposed to Stx2a (10 ng/ml) for 9 h (n = 3 biological replicates).
  3. Real‐time qRT‐PCR analyses of fold changes in transcript levels for each cytokine/chemokine gene in D‐THP‐1 cells following Stx2a (10 ng/ml) intoxication for various time points in the presence or absence of OSMI‐1 (10 µM, final). Data are normalized using GAPDH as a loading control (n = 3 biological replicates).

Data information: For graphs in (B and C), error bar represents mean ± SEM. Statistical analysis was performed using two‐tailed Student’s t‐test. *P < 0.05; **P < 0.01; and ***P < 0.001. The effects of OSMI‐1 were compared with those of the vehicle (DMSO) control at each time point.

Source data are available online for this figure.