Table 1.

Analysis of the molar mass distribution of ICs from AF4 data.

Sample	Range of assigned molar masses (kDa)			Mass‐weighted mean of assigned molar masses (kDa)
	Run 1	Run 2	Run 3	Run 1	Run 2	Run 3	Mean ± SD
Infliximab, IFX	158–182	153–164	159–193	162	156	163	160 ± 4
TNF‐alpha	52–55	51–61	52–62	52	52	52	52 ± 0
ICs
IFX/TNF 3:1	182–1.16 × 103	164–1.11 × 103	193–1.10 × 103	409	398	518	442 ± 66
IFX/TNF 1:1	182–2.06 × 103	164–1.31 × 103	193–1.42 × 103	801	589	681	690 ± 106
IFX/TNF 1:3	182–5.05 × 103	164–6.89 × 103	193–10.8 × 103	1.77 × 103	2.00 × 103	2.61 × 103	2.13 × 103 ± 435
IFX/TNF 1:9	182–5.36 × 103	164–3.38 × 103	193–3.51 × 103	1.66 × 103	1.18 × 103	1.17 × 103	1.34 × 103 ± 279
IFX/TNF 1:27	182–1.68 × 103	164–768	193–1.01 × 103	689	464	521	558 ± 117

For a given elution time, the AF4 profiles provide the concentration (UV) at which a given molar mass (MALS) of a protein is present in the sample. The molar mass distribution of Ifx, TNF‐α and their immune complexes (sICs) was obtained by plotting the cumulative frequency as a function of molar mass. For a selected range of molar masses, a mass‐weighted mean value (<M_w>) was calculated. All detected molar masses were selected in the case of Ifx and TNF‐α whereas only molar masses larger than the maximal molar mass found for Ifx were assigned to sICs. The table shows the range of assigned molar masses and the calculated <M_w> for each AF4 run (n = 3).