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. 2021 Dec 13;14(1):e14502. doi: 10.15252/emmm.202114502

Figure EV5. Carfilzomib synergizes with PD‐1 inhibitors to treat lung cancer.

Figure EV5

  • A
    Gating strategy was used for analyzing the infiltration of CD8+ or CD4+ T cells in lung tissues. After gating the single (by FSC‐A and SSC‐W) and living cells (DAPI negative), CD8‐ or CD4‐positive cells were gated on CD45+ cells.
  • B, C
    Elimination of CD8+ T cells by CD8α antibody. After 2‐week treatment with antibody, orbital blood was collected and red blood cells were lysed. CD8+ T cells were analyzed by flow cytometry (B). Statistics on percentages of CD8‐positive cells (C). Data are means ± SD of three independent experiments. **P < 0.01 (Student’s t‐test).
  • D, E
    Elimination of CD4+ T cells by CD4 antibody. After 2‐week treatment of antibody, orbital blood was collected and red blood cells were lysed. CD4+ T cells were analyzed by flow cytometry (D). Statistics on percentage of CD4‐positive cells (E). Data are means ± SD of three independent experiments. ***P < 0.001 (Student’s t‐test).
  • F–I
    Statistics on tumor burden of mice before and after treatment. Data are means ± SD of three independent experiments (F, H). Statistics on relative tumor area and Ki67+ cells in sections of lung tissue of mice (G, I). Data are means ± SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 (Student’s t‐test).