Table 1. Number of Copies Per Cella (×104) in HeLa Cells Determined by MS Western,22 MBAQ, Proteomics Ruler,32 and iBAQ31.
| MS Western |
MBAQ | Proteomics
Ruler |
iBAQ | |||
|---|---|---|---|---|---|---|
| protein | Kumar et al. (2018) | Raghuraman et al. (this work) | Raghuraman et al. (this work) | Hein et al. (2015) | Itzhak et al. (2016) | Nagaraj et al. (2011) |
| PLK-1 | n.a.b | 6.8 | 5.4 (20%)c | 13 (91%) | 16 (135%) | 3.7 (46%) |
| CAT | 100 | 149 | 113 (24%) | 17 (88%) | 87 (42%) | 23 (84%) |
| TBA1A | 6926 | 8166 | 8162 (0.05%) | n.q.d | n.q. | 32 (99%) |
| GAPDH | n.a. | 15 546 | 14 692 (5.4%) | 1747 (89%) | 11 848 (24%) | 1600 (89%) |
a
Copy numbers were rounded up.
b
n.a., not available.
c
Error in quantification (in %) calculated relative to MS Western quantities (Raghuraman et al., this work) taken here as true values.
d
n.q., not quantified.