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. 2022 Jan 4;14(1):243. doi: 10.3390/cancers14010243

Figure 5.

Figure 5

O-GlcNAcylation in the N-terminal region of SAM68 and its functional significance. (A–C) Two different KHDRBS1-targeting shRNAs (shSAM68 #1 and #2) were used to generate independent SAM68-knockdown CL1-5 clones. The LKO vector was used as an infection control (CTL). Cells were subjected to Western analysis (A), Transwell migration assays (B), and Transwell invasion assay (C). (D–F) CL1-5 shSAM68 #2 cells were transfected by the control vector or a construct to express Flag-tagged SAM68 WT or mutant 6A, and resulting cells were subjected to Western analysis (D), Transwell migration assays (E), and Transwell invasion assays (F). All quantitative data shown are the means ± SD of multiple independent experiments; *, p < 0.05; **, p < 0.01. Scale bar, 100 μm. Detailed information about the Western blotting can be found in Figure S13.