Figure 5.

Genes associated with melanoma progression show significant enrichment of multiple pathways related to the immune system. Pathway enrichment was performed using Reactome’s over-representation analysis on a combination of genes identified by the following analyses: (1) top ten differentially expressed gene in melanoma progression in at least one dataset (Figure 1); (2) gene identified in at least one binomial regression model distinguishing nevi vs. cutaneous malignant melanoma (CMM) (Figure 2C–E); and (3) a significantly differentially expressed gene between nevi and CMM in at least two datasets (Figure 3). Genes colored in red and bolded represent genes that were differentially expressed between nevi and CMM in at least two datasets (Figure 3). A pathway was considered to be enriched if it had a Benjamini–Hochberg-adjusted p-value < 0.05 (corresponding to a false discovery rate < 5%). The eleven significantly enriched pathways identified are each represented as a circle, with nested circles indicating pathways that are subsets of one another. IL-10, interleukin-10, IL-4, interleukin-4, IL-13, interleukin-13.