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. 2021 Dec 13;10(12):1985. doi: 10.3390/antiox10121985

Figure 5.

Figure 5

ROS formation kinetics in HeLa cells. Control cells were stained with DCFH-DA and irradiated. Two identical plates were treated and irradiated with XL-8 (100 nM) and XL-8-NPs (100 nM). Images were acquired every 30 min. Captured images from IncuCyte using a ×20 objective; 200 μm scale bars (A). At the end of analysis, an automatic real-time assessment was performed using IncuCyte, measured as green object integrated intensity for all cells stained green with DCFH-DA, allowing generating of graphical data (B). Fluorescent images of HeLa cells. Subcellular localization was investigated after treatment with XL-8 (100 nM) and XL-8-NPs (120 nM), hoechst 33,342, DCFH-DA, and MitoSox Red (C).