Figure 3.

Evaluation of tumor engraftment and growth kinetics. (A) Longitudinal quantification of the bioluminescence (full lines) and circulating blast frequency (dotted lines) of two representative animals. (B) Corresponding bioluminescence images of mice depicted in Figure 3A. (C) Influence of serial transplantation on the engraftment velocity. Samples with BCR::ABL1, KMT2A and T-ALL are painted in green, red and grey, respectively. Flow cytometric determination of the tumor cell frequency in peripheral blood was performed throughout the observation period. Each line represents an individual animal, and mice engrafted within the same passage are displayed in the same color. Earlier passages are depicted in darker colors. A line printed in bold indicates that the following passage was derived from the indicated animal. (D) Flow cytometric analysis of bone marrow (BM) and spleen infiltration upon termination of the experiment. Each dot represents an individual mouse. Mean ± standard deviation.