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. 2022 Jan 3;11(1):150. doi: 10.3390/cells11010150

Table 2.

All variants detected during the serial transplantation of KMT2A-positive primary samples.

#0054 #0094 #0122 #0134 #0152 #0159
Gene Base Change AA Change Prim P1 Prim P1 P2 P3 P4 Prim P1 P2 P3 Prim P1 P2 P3 Prim P1 P2 Prim P1 P2 P3
APC c.4326T > A p.(=) 0 0 51 45 45 45 46 0 0 0 48 48 45 0 0 0 0 0 0 0
0 0 45 45
APC c.4479G > a p.(=) 0 0 100 100 100 100 100 49 52 50 100 99 100 100 100 100 0 0 0 0
50 51 100 100
ATM c.*29C > G p.? 0 0 0 0 0 0 0 0 0 0 0 0 0 0 49 50 50 53
0 0 0 0 0 0
ATM c.5793T > C p.(=) 0 0 0 0 0 0 0 0 0 0 0 0 0 0 52 50 50 51
0 0 0 0 0 0
BRAF c.1789C > T p.(=) 47 52 51 49 47 0 0 0 51 48 49 0 0 0 0 0 0 0
0 0 0 0 46 50
CSF1R c.*36CA > TC p.? 100 100 100 100 100 100 100 34 29 31 100 100 100 36 31 28 100 100 100 100
13 30 100 100
EGFR c.2361G > A p.(=) 51 51 100 100 100 100 100 53 44 52 100 100 100 100 100 100 51 49 49 48
48 47 100 100
ERBB4 c.421+58A > G p.? 59 59 0 0 0 0 0 100 100 100 0 0 0 0 0 0 0 0 0 0
100 100 0 0
ERBB4 c.884-20T > C p.? 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
0 0 0 0
FBXW7 c.1524A > G p.(=) 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 0
0 0 0 0
FGFR3 c.1953G > A p.(=) 100 100 0 0 0 0 0 100 100 100 100 100 100 0 0 0 0 0 0 0
100 100 100 100
FGFR3 c.1959+22G > A p.? 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
49 50 0 0
FLT3 c.1310-3T > C p.? 100 100 100 100 100 100 100 0 0 0 100 100 100 100 100 100 51 52 52 52
0 0 100 100
FLT3 c.1775T > C p.Val592Ala 0 0 44 50 51 51 49 0 0 0 51 52 49 0 0 0 0 0 0 0
0 0 56 52
FLT3 c.2504A > T p.As p835Val 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 0
0 0 0 0
HRAS c.81T > C p.(=) 0 0 60 49 54 48 48 52 49 52 48 53 51 55 55 46 0 0 0 0
50 51 50 50
IDH1 c.315C > T p.(=) 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 53 53 52
0 0 0 0
JAK3 c.2164G > A p.Val722le 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 49 49 52
0 0 0 0
KDR c.798+54G > A p.? 0 0 52 44 48 45 47 49 50 45 46 48 52 100 100 100 100 98 98 100
48 46 50 51
KDR c.4008C > T p.(=) 0 0 0 0 0 0 0 49 53 48 0 0 0 0 0 0 0 0 0 0
49 48 0 0
KIT c.1638A > G p.(=) 51 50 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
0 0 0 0
KRAS c.34G > A p.Gly 12Ser 0 0 0 0 0 0 0 31 24 4 0 0 0 0 0 0 0 0 0 0
6 7 0 0
MET c.3029C > T p.Thr 1010lle 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 50 50 50
0 0 0 0
NRAS c.183A > C p.Gln61His 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0
0 0 0 0
PDGFRA c.1701A > G p.(=) 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100
100 100 100 100
PDGFRA c.2472C > T p.(=) 49 47 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
0 0 0 0
PIK3CA c.352+40A > G p.? 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 53 53 51
0 0 0 0
PIK3CA c.1636C > A p.Gln546Lys 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 0 0 0
0 0 0 0
PIK3CA c.3075C > T p.(=) 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 50 50 52
0 0 0 0
RET c.2307G > T p.(=) 48 51 54 49 52 54 50 0 0 0 51 50 54 54 50 49 100 100 100 100
0 0 51 52
RET c.2712C > G p.(=) 0 0 0 0 0 0 0 0 0 0 0 0 0 51 50 50 51 47 47 49
0 0 0 0
SMAD4 c.955+58C > T p.? 54 49 48 51 54 54 56 0 0 0 53 53 48 0 0 0 0 0 0 0
0 0 51 48
STK11 c.126G > C p.(=) 0 0 0 0 0 0 0 0 0 0 5 0 0 0 0 0 0 0 0 0
0 0 0 0
STK11 c.465-51T > C p.? 100 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 100 100 100
0 0 0 0
TP53 c.215C > G p.Pro72Arg 99 100 94 49 49 50 50 100 100 100 52 53 53 100 100 99 56 52 52 49
98 99 51 61
TP53 c.847C > A p.Arg283Ser 0 0 86 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
0 0 0 0

All variants detected are summarized irrespectively of their pathogenicity. Numbers indicate the allele frequency (%). AA, amino acid; p.(=), no change in protein translation; p.?, extraexonic variant; Prim, primary tumor; P, passage. The color of the boxes indicates the allele frequency, with low frequencies displayed in red and high frequencies in blue shade. For patients #0122 and #0134, two individual mice were analyzed in passage 2. The allele frequencies of these animals are listed on top of each other. The cells of the second (lower) mouse were used for xenotransplantation and the generation of passage 3.