Figure 3.

Responses of Dpt and BdPGRPs to opportunistic pathogen E. coli challenges. Relative expression of Dpt (A), BdPGRP-LB (B), BdPGRP-SB₁ (C), and BdPGRP-SC₂ (D) after infection with E. coli at different time points, respectively. The data are expressed as mean ± SEM and the mean refers to the average of four biological replicates for each sample. Statistical analysis was based on Student’s t-test. * p < 0.05; ** p < 0.01; *** p < 0.001. The relative gene expression data were analyzed using a 2^−ΔΔCT method and the data were normalized to reference gene Rpl32.