Table 1.

Primers used in RT-PCR and qRT-PCR.

Primer	Sequence (from 5′ to 3′)	Purpose
PGRP-SC2 5′RACE outer	CCTTAGCGGCAGCAATCT	RACE
PGRP-SC2 5′RACE inner	CCACGACCCTCATACACT	RACE
PGRP-SC2 3′RACE outer	GCAAGTGTATGAGGGTCG	RACE
PGRP-SC2 3′RACE inner	TTACTGCTCCACCCAAAC	RACE
QPGRP-LB F	GCGTGGCTGGAATGACATTG	qRT-PCR
QPGRP-LB R	CGGTCATTGTATTTGGGCGC	qRT-PCR
QPGRP-SB F	TGGCATTGTCTTCATCGGCA	qRT-PCR
QPGRP-SB R	CAGATAACCCTTTTGCACCGC	qRT-PCR
QPGRP-SC2 F	GGGTCGTGGTTGGAGTACAG	qRT-PCR
QPGRP-SC2 R	GATCTGAGCGGCTGTTGGAA	qRT-PCR
QRpL32 F	CCCGTCATATGCTGCCAACT	qRT-PCR
QRpL32 R	GCGCGCTCAACAATTTCCTT	qRT-PCR
QDiptericin F	GCATAGATTTGAGCCTTGACACAC	qRT-PCR
QDiptericin R	GCCATATCGTCCGCCCAAAT	qRT-PCR
PGRP-LB T7F	GGATCCTAATACGACTCACTATAGGATGCCCAGCGCCTGTTAC	dsRNA synthesis
PGRP-LB T7R	GGATCCTAATACGACTCACTATAGGTGCGGCCACGTCGTAATC	dsRNA synthesis
PGRP-SB T7 F	GGATCCTAATACGACTCACTATAGGTGTTTTGCGCTCAGGATCCA	dsRNA synthesis
PGRP-SB T7R	GGATCCTAATACGACTCACTATAGGTGGCCCAGCAGTGTGTAATT	dsRNA synthesis
PGRP-SC2 T7 F	GGATCCTAATACGACTCACTATAGGGGCTTTCAAGACTTTCCTC	dsRNA synthesis
PGRP-SC2 T7R	GGATCCTAATACGACTCACTATAGGAACCACGACCCTCATACAC	dsRNA synthesis
EGFP T7L	GGATCCTAATACGACTCACTATAGGACGTAAACGGCCACAAGTTC	dsRNA synthesis
EGFP T7R	GGATCCTAATACGACTCACTATAGGAAGTCGTGCTGCTTAATGTG	dsRNA synthesis

Primers starting with Q were used for qRT-PCR; the underlined sections indicate T7 polymerase promoter.