Figure 5.
C-ion irradiation moderately changes the mRNA expression of anti-oxidative factors, ROS and adhesion of HMVEC cells under static conditions. (A) Experimental time course of analysis of C-ion irradiation-induced changes of the anti-oxidative defense of HMVEC, cultured under normal static conditions. (B) Relative mRNA expression of Nrf2, SOD1, GPx1 and Catalase at 24 h after C-ion irradiation (mean + SEM; n = 8; * p < 0.05, Kruskal-Wallis test vs. 0 Gy). (C) Intracellular ROS content, relative to non-irradiated cells, after C-ion irradiation of HMVEC with indicated doses (mean + SEM; n = 7–8). (D) Experimental setup of adhesion assays, performed 24 h after C-ion exposure. (E) Relative mRNA expression of indicated adhesion molecules after irradiation with C-ions (mean + SEM; n = 8; * p < 0.05, Kruskal-Wallis test vs. 0 Gy). (F) Relative PBL adhesion at 24 h after irradiation of HMVEC cells with C-ions (mean + SEM; n = 2–6).