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. 2022 Jan 3;11(1):149. doi: 10.3390/cells11010149

Figure 5.

Figure 5

TGF-β-mediated downregulation of NPNT is dependent on the non-canonical signaling pathways in podocytes. (a,b) Western blot to determine NPNT protein expression in cultured human podocytes in the presence of (a) canonical (SM16 and SIS3) or (b) non-canonical (PD169316, SP600125, PD98059, AKT inhibitor, MG-132, and Y-27632) pathway inhibitors after stimulation with TGF-β. NPNT protein expression, normalized to GAPDH, is given as the change compared to stimulation with inhibitor without TGF-β. Representative Western blots are shown for each treatment. n = 5–6 independent experiments; * p < 0.05.