Table 2.
Primer and probe sequences used in real-time methylation-specific PCR assays.
| Gene | Primer Sequences 5′-3′ (For SB–Converted Sequences) |
Detection | PCR Product, bp |
|---|---|---|---|
| BRCA1 | F: TCGTGGTAACGGAAAAGCGC R: AAATCTCAACGAACTCACGCCG |
LC green | 75 |
| CST6 | F: TCGAGTTTCGTTTTATTTTAGGTC R: CATAACCGTCAATACCGTCG |
FAM–TAGCGGGTAAAAGTGCGCGGTCTAAGTT–BBQ | 134 |
| MGMT | F: TTTCGACGTTCGTAGGTTTTCGC R: CGTTTCGTTTTCGGAAGAGTGC |
LC green | 81 |
| RASSF10 | F: CGTCGTTTTAGTAGATTTCGGTC R: CGTCGAAACAAATAATACGACG |
LC green | 143 |
| SLFN11 | F: ATGGAGGGAGCGAGGAGAC R: AACGAATCTACTAAACCCGCG |
LC green | 155 |
| USP44 | F: TATTTGTAGTGTCGTCGGGATAC R: GCGTTTCTACCAAACAATTCG |
LC green | 143 |