Table 3.
Antibodies used to characterize the induced pluripotent cell line Mad6. Antibodies produced by R&D Systems, Minneapolis, MN, USA, Merck KGaA, Darmstadt, Germany and Thermo Fisher Scientific.
| Purpose | Antibody | Dilution | Company and Cat# |
|---|---|---|---|
| Differentiation markers | SOX17 PE (Endoderm) | As per datasheet | R&D ICI9241P |
| OTX2 AF488 (Ectoderm) | As per datasheet | R&D ICI979G | |
| BRACHYURY APC (Mesoderm) | As per datasheet | R&D IC2085A | |
| Pluripotency markers | Mouse anti-OCT4 | 1:400 | Merck, MAB4401 |
| Goat anti-NANOG | 1:100 | R&D Systems, AF1997 | |
| Mouse anti-SSEA4 | 1:400 | Merck, MAB4304 | |
| Mouse anti-TRA-1-81 | 1:200 | Merck, 4381 | |
| Secondary antibodies | Goat anti-mouse Alexa Fluor 488 | 1:300 | Thermo Fisher Scientific, A11001 |
| Goat anti-mouse Alexa Fluor 568 | 1:300 | Thermo Fisher Scientific, A11004 | |
| Donkey anti-goat Alexa Fluor 568 | 1:300 | Thermo Fisher Scientific, A11057 |