Skip to main content
. 2022 Jan 5;14(1):252. doi: 10.3390/cancers14010252

Figure 1.

Figure 1

CD36 promotes invasion and colony formation in HCT116 cells. (A) Matrigel trans-well invasion assay. Fold change of the invaded cells/field, absorbance at 560 nm of the de-stained Matrigel trans-well invasion chambers and raw images of the HCT116 NTC and CD36 shRNA #1 and #2 cell lines are shown. (B) q-RT-PCR and Western blot analyses demonstrating the levels of CD36 knockdown in HCT116 NTC/CD36shRNAs cells. (C) Matrigel trans-well invasion assay. Average count of invaded cells/field, absorbance at 560 nm of the de-stained Matrigel trans-well invasion chambers and raw images of the HCT116 p-Lenti-Control and p-Lenti-CD36 overexpression cell lines are shown. (D) Quantification and representative images of the HCT116 p-Lenti-Control and p-Lenti-CD36 overexpression soft agar colony formation assays. (E) q-RT-PCR demonstrating the levels of CD36 overexpression in the HCT116 p-Lenti-Control and p-Lenti-CD36 overexpression cell lines. All functional assays were performed at least 3 times using multiple replicates. * p < 0.05, ** p < 0.01, *** p < 0.001, SEM.