Skip to main content
. 2021 Dec 28;11(1):86. doi: 10.3390/cells11010086

Figure 1.

Figure 1

The viability of TCS on HTR-8/SVneo and JEG-3 cells was detected by Cell Counting Kit-8 assay for 24 h. (A) Cell vitality in HTR-8/SVneo and JEG-3 cells exposed to TCS. (B) Expression of PPARγ was determined by RT-PCR in HTR-8/SVneo and JEG-3 cells exposed to indicated TCS for 24 h. (C) Cell vitality was detected when exposed to TCS (40 µM for HTR-8/SVneo, 30 µM for JEG-3) in the absence or presence of rosiglitazone or GW9662 in the two cell lines. PPARγ was overexpressed (D) and knockdown (E) in the two cell lines and co-treated with TCS in HTR-8/SVneo and JEG-3 cells while cell vitality was analyzed. (F) HTR-8/SVneo and JEG-3 cells exposed to TCS while in the absence or presence of rosiglitazone (10 µM) and GW9662 (10 µM). HTR-8/SVneo and JEG-3 cells exposed to TCS during PPARγ overexpression (G) or knockdown (H). The data are shown as the means ± S.E.M. * p < 0.05; ** p < 0.01; compared with the indicated group, n = 3.