The gene and protein expression of PPARγ-regulated genes in placentas of gestational mice exposed to TCS. (A) The PPARγ expression level was detected by RT-PCR in GD17.5 mice placentas. (B,C) The mRNA expression of cell growth, angiogenesis, migration (B), and inflammation (C)-related genes in the placenta of GD17.5 mice treated with indicated doses of TCS and with or without rosiglitazone. (D) The protein expression of ANGPTL4, MMP-2, and IL-1β were analyzed by Western blot in placenta of GD17.5 mice treated with or without TCS (100 mg/kg/day). The relative values of protein expression are shown on the right. The data are shown as the means ± S.E.M. * p < 0.05; ** p < 0.01; compared with the indicated group, n = 8.