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. 2021 Dec 14;10(12):1986. doi: 10.3390/antiox10121986

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Prdm16 capable of activating Ucp1 promoter (A) Effect of Prdm16 on Ucp1 promoter (double luciferase report gene method) (B) PCR electrophoresis diagram and sequencing comparison diagram of pLacZi-Ucp1 bacterial solution. M: Kang Wei 200B PLA DD;Lane 1–4: PCR products from the picked four single-colony bacterial solutions. (C) PCR electrophoresis diagram and sequencing comparison diagram of pJG-Prdm16 bacterial solution. M: Kang Wei 200B PLA DD; Lane 1–4: PCR products from the picked four single-colony bacterial solutions. (D) Effect of Prdm16 on Ucp1 promoter (yeast one-hybrid assay) (E) Clone electrophoretic images of Ucp1 promoter with different lengths. (F) PGL3-Basic enzyme digestion electrophoresis diagram (G) PCR electrophoresis map of bacterial solution after ligation of Ucp1 promoter with different lengths to pGL3-Basic. (H) Effects of Prdm16 on promoters of different fragments Ucp1. Compared with control, ** means p < 0.01; *** means p < 0.001.