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. 2021 Dec 14;10(12):1986. doi: 10.3390/antiox10121986

Figure 5.

Figure 5

PPARα binds to Ucp1 promoter to activate transcription. (A) Cloned PPARα electrophoresis M: Kang Wei 200B PLA DD; Lane 1–7: PPARα amplification product. (B) Electrophoresis map of pCMV-N-Flag enzyme digestion product, M: Kang Wei 200B PLA DD; Lane 1–3: pCMV-N-Flag double enzyme digestion product. (C) PCR electrophoresis diagram of pCMV-PPARα bacterial solution;M: Kang Wei 200B PLA DD; Lane 1–11: PCR products from picked 11 single-colony bacterial solutions (D) Comparison of pCMV-PPARα sequencing results. (E) Effect of PPARα on the intact Ucp1 promoter (−2000 bp). (F) Effect of PPARα on promoter of Ucp1 with different fragments. ** mesns p < 0.01.