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Detection of nPru p 3 spiked in 10% Fruktozym-treated apple-derived pectin (AP) (a), or citrus-derived pectin (CP) (b) via immunoblotting. The spiking concentrations represent the nsLTP thresholds eliciting anaphylactic (3 µg/mL) or oral symptoms (0.1 µg/mL). Detected bands were analyzed semi-quantitatively as area under the curve (AUC) normalized to blank.
