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. 2021 Dec 23;11(1):23. doi: 10.3390/foods11010023

Figure 3.

Figure 3

Lf alleviates AFM1-induced autophagy in NCM460 cells. 100 μg/mL Lf and 8 μg/mL AFM1 were treated in NCM460 cells alone or in combination. (A) Relative mRNA levels of autophagy genes. (B,C) Immunoblotting was performed on protein extracts for autophagy proteins and band densities quantification. (D)The changes of LC3 positive level were analyzed by confocal microscope. Results represented as means ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. control (C/CTL). # p < 0.05, ## p < 0.01, ### p < 0.001 vs. AFM1.