Spontaneous physical activity induces a decrease in the immune infiltrate to the benefit of anti-inflammatory and cytotoxic cells. (A) Global immune infiltrate in the tumours, results are in cells per mg of tumour (B) Pro-/anti-tumour immune infiltrate balance in tumour with results in cells per mg of tumour. (C) Immune phenotyping of the immune infiltrate in tumour of standard group, with results in % of immune live cells. (D) Immune phenotyping of the immune infiltrate in tumour of enriched group, with results in % of immune live cells. Data are expressed as mean ± SEM (n = 5 mice tumour phenotyping analysis/group). Data were analysed by Mann–Whitney t-test. Representative data are shown: * p < 0.05, SE vs. EE. Representative data are shown for a and b, and means without a common letter on a same line are significantly different, SE vs. EE.