Table 3.
Phase | Gene | Adjusted p Value (*) | Adjusted p Value (cn) | Ct Mean No Metastasis ± SD |
Ct Mean Metastasis ± SD |
Copies Per µL Mean No Metastasis ± SD | Copies Per µL Mean Metastasis ± SD |
---|---|---|---|---|---|---|---|
B | HV1-short | 0.020 | 0.069 | 24.54 ± 4.18 | 22.06 ± 2.08 | 2.38 ± 3.77 | 2.55 ± 2.04 |
HV1-long | 0.035 | 0.133 | 23.92 ± 4.61 | 21.35 ± 2.56 | 2.67 ± 3.92 | 2.56 ± 1.72 | |
CYB-short | 0.078 | 0.223 | 25.71 ± 4.29 | 23.69 ± 2.54 | 2.08 ± 3.11 | 1.90 ± 1.35 | |
HBB-long | 0.020 | 0.029 | 33.52 ± 2.99 | 36.09 ± 2.69 | 1.07 ± 2.02 | 0.09 ± 0.16 | |
C | CYB-short | 0.359 | 0.037 | 19.95 ± 2.73 | 20.67 ± 2.03 | 24.36 ± 29.66 | 10.18 ± 7.37 |
HBB-short | 0.001 | 0.002 | 19.18 ± 2.41 | 21.24 ± 0.89 | 1092.33 ± 1254.73 | 194.36 ± 102.38 | |
HBB-long | 0.006 | 0.012 | 30.37 ± 3.71 | 32.99 ± 1.55 | 23.39 ± 49.39 | 0.38 ± 0.48 | |
D | HBB-long | 0.001 | 0.001 | 31.84 ± 3.48 | 36.03 ± 2.77 | 7.39 ± 15.66 | 0.07 ± 0.09 |
F | HBB-long | 0.007 | 0.014 | 31.72 ± 2.13 | 33.44 ± 1.34 | 2.22 ± 4.42 | 0.22 ± 0.19 |
Ct: cycle threshold; CYB: Apocytochrome B; HBB: Hemoglobin subunit beta; HV1: Hypervariable region 1; SD: Standard deviation. HBB as nuclear marker vs. HV1 and CYB as mitochondrial markers. (*) Just FDR adjusted p values comparing genes in cases vs. controls by qPCR analysis. Adjusted p values (cn) represent values of comparisons cases vs. controls in copy number by dPCR analysis. The italics indicates significant p values.