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. 2021 Dec 1;10(12):1934. doi: 10.3390/antiox10121934

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Western blot analyses in the samples of rat optic nerve (ON) post different operations. (A) Representative photograph of the immunoblotting of the expression of p-Akt1, Nrf2, Sirt3, and HO-1 in the ON samples. (B) Quantification of the protein bands of p-Akt1, Nrf2, Sirt3, and HO-1. Each value was measured by using iBright imaging software and normalized to GAPDH. The expressions of p-Akt1, Nrf2, Sirt3, and HO-1 were significantly higher in the PEG-G-CSF-treated crushed samples than those in the sham group or the PBS-treated crushed samples. Data are expressed as mean ± standard deviation (SD) in each group (n = 6 in each group). *: p < 0.05. **: p < 0.01.