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. Author manuscript; available in PMC: 2022 Jan 11.
Published in final edited form as: Mol Cell. 2021 Dec 9;82(1):140–158.e12. doi: 10.1016/j.molcel.2021.11.016

Figure 5. The stable topoisome has specific composition and stoicheometry.

Figure 5.

(A) HeLa cell nuclear extracts was eluted stepwise with an increasing KCl concentration and examined for the indicated proteins. A mix of recombinant proteins identified the migration of each protein.

(B) The P11 1 M eluate was further fractionated, and every other fraction from 16–44 co-elution was examined. Protein mix as in (A).

(C) Proteins were mixed as shown, IP with anti-MYC, and probed for TOP2A, TOP2B, TOP1 and MYC.

(D) Indicated proteins were mixed, IP with anti-MYCN, and probed as in (C).

(E and F) Full-length MYC or MYCN were pre-incubated with TOP2A (E) or TOP2B (F) before the addition of kDNA and decatenation assay.

See also Figure S5 and Table S4.