Table 2.
List of used antibodies for immunohistochemical staining and Western blotting.
| Primary Antibody | Host | Dilution | Supplier/Reference | |
|---|---|---|---|---|
| Cre recombinase | Mouse | 1/500 | Frozen | Euromedex (CRE-2D8-As) |
| GFAP | Rabbit | 1/10,000 | NDF | Dako (Z0334) |
| 4-HNE | Rabbit | 1/100 | 1% PFA | Calbiochem (393207) |
| MFP2 | Rabbit | 1/200 | Western blotting | Proteintech (15116-1-AP) |
| Opsin | Rabbit | 1/100 | NDF | Millipore (AB5405) |
| Peanut agglutinin | / | 1/100 | NDF | Vector Laboratories (FL-1071) |
| Phalloidin-Alexa647 | / | 1/100 | RPE flatmount | Invitrogen (A30107) |
| PKCα | Mouse | 1/100 | 1% PFA | Santa Cruz (SC-17769) |
| PLIN2 |
Rabbit |
1/1000 1/200 |
NDF RPE flatmount |
NOVUS (NB110-40877) |
| Rhodopsin | Mouse | 1/1000 (Alexa 1/750) | NDF | Millipore (MAB5356) |
| Thiolase | Rabbit | 1/500 | Western blotting | Van Veldhoven P.P. [35] |
| VGLUT1 | Rabbit | 1/1000 | 1% PFA | Synaptic Systems (135303) |
| Vinculin | Mouse | 1/2000 | Western blotting | Sigma (V9131) |
GFAP—glial fibrillary protein; 4-HNE—4-hydroxy-2-nonenal; MFP2—multifunctional protein 2; PKCα—protein kinase Cα; PLIN2—perilipin 2; VGLUT1—vesicular glutamate transporter 1.