Table 5.

Relative mRNA Expression in miR-200a Overexpression Background

Gene Symbol	Gene Description	Ad-miR-200a to Ad
Do novo fatty acid synthesis
FASN	Fatty acid synthase	1.38*
ACACA	Acetyl-coenzyme A carboxylase α	1.47**
Triglyceride synthesis
SCD	Stearoyl-CoA desaturase (delta-9-desaturase)	1.04
GPAM	Glycerol-3-phosphate acyltransterase	1.11
AGPAT6	1-Acylaglycerol-3-phosphate O-acyltransferase 6	0.92
LPIN1	Lipin 1	0.15
DGAT1	Diacylglycerol acyltransferase 1	1.52*
Lipid drop formation and secretion
ADFP	Adipose differentiation-related protein	0.49*
TIP47	PAT-related proteins family, member 47	0.11*
BTN1A1	Goat butyrophilin, subfamily 1, member A1	0.97
Fatty acid uptake
CD36	CD 36 molecule (thrombospondin receptor)	1.08*
SLC27A6	Solute carrier family 27 transporter, subfamily A, member 6	1.49*
FA transport
ABCA1	ATP-binding cassette, subfamily A, member 1	0.85*
ABCG1	ATP-binding cassette, subfamily G, member 1	0.41
FA intracellular transport
FABP3	Fatty acid-binding protein 3	1.81*
Fatty acids receptor
GPR41	Orphan G protein-coupled receptor family, member 41	0.59*
Transcription factor
SREBP-1c	Sterol regulatory element-binding protein, member 1c	1.15*
PPARγ	Peroxisome proliferator-activated receptor, member γ	1.28*
LXRα	Nuclear oxysterol receptor, member α	0.81**

Genes were clustered based on main functions relative to milk fat synthesis. Ad-infected cells were used as control for Ad-miR-200a-infected cells. MRNA expression levels were determined at 72 h postinfection in goat mammary gland epithelial cells. mRNA levels were measured by qRT-PCR, normalized to GAPDH and plotted relative to controls. Data are presented as mean ± SD (n = 18).

*p < 0.05, **p < 0.01.