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. 2013 Dec 3;16(1):1–13. doi: 10.3727/105221613X13776146743262

Table 5.

Relative mRNA Expression in miR-200a Overexpression Background

Gene Symbol Gene Description Ad-miR-200a to Ad
Do novo fatty acid synthesis
FASN Fatty acid synthase 1.38*
ACACA Acetyl-coenzyme A carboxylase α 1.47**
Triglyceride synthesis
SCD Stearoyl-CoA desaturase (delta-9-desaturase) 1.04
GPAM Glycerol-3-phosphate acyltransterase 1.11
AGPAT6 1-Acylaglycerol-3-phosphate O-acyltransferase 6 0.92
LPIN1 Lipin 1 0.15
DGAT1 Diacylglycerol acyltransferase 1 1.52*
Lipid drop formation and secretion
ADFP Adipose differentiation-related protein 0.49*
TIP47 PAT-related proteins family, member 47 0.11*
BTN1A1 Goat butyrophilin, subfamily 1, member A1 0.97
Fatty acid uptake
CD36 CD 36 molecule (thrombospondin receptor) 1.08*
SLC27A6 Solute carrier family 27 transporter, subfamily A, member 6 1.49*
FA transport
ABCA1 ATP-binding cassette, subfamily A, member 1 0.85*
ABCG1 ATP-binding cassette, subfamily G, member 1 0.41
FA intracellular transport
FABP3 Fatty acid-binding protein 3 1.81*
Fatty acids receptor
GPR41 Orphan G protein-coupled receptor family, member 41 0.59*
Transcription factor
SREBP-1c Sterol regulatory element-binding protein, member 1c 1.15*
PPARγ Peroxisome proliferator-activated receptor, member γ 1.28*
LXRα Nuclear oxysterol receptor, member α 0.81**

Genes were clustered based on main functions relative to milk fat synthesis. Ad-infected cells were used as control for Ad-miR-200a-infected cells. MRNA expression levels were determined at 72 h postinfection in goat mammary gland epithelial cells. mRNA levels were measured by qRT-PCR, normalized to GAPDH and plotted relative to controls. Data are presented as mean ± SD (n = 18).

*p < 0.05, **p < 0.01.