Figure 3.

Effects of ATG7 and p62 depletion on TRAIL-induced Ca²⁺ influx in NB4 cells. NB4 cells were depleted for ATG7 (A) and p62 (B) as mentioned in the Materials and Methods section. ATG7 knocked-out (KO) NB4 cells were generated by CRISPR/Cas9 system using sgATG7 #1–3 and sgcontrol lentivirus vectors. For p62/SQSTM silencing, two lentiviral vectors specifically expressing shRNA p62 #1 and shRNA p62 #2 were used. (C,D) ATG7-depleted NB4 cells and NB4 cells expressing shp62 were exposed to 250 ng/mL TRAIL and relative [Ca²⁺]i levels were evaluated via fluorescence cell imaging after the Fura-2AM-staining of cells.