Figure 2.

Effect of NAM manipulation on antioxidant ex vivo protection conferred by plasma isolated HDL. (a) Representative diene formation curves of human LDL incubated with mouse HDL isolated from untreated and NAM-treated wildtype C57BL/6J mice in the presence of 2.5 μM CuSO₄ at 37 °C. The final oxidation kinetics of the LDL+HDL mixture was shown after subtracting the kinetics of HDL incubated without LDL. (b) HDL antioxidant activity against LDL oxidative modification. Methods used can be found in greater detail in reference [84]. Data were expressed as relative lag phase to LDL oxidized in the presence of HDL isolated from Untreated mice (arbitrary unit = 1). Data were expressed as the median ± interquartile range (n = 4–5 independent pools per group). Differences between the mean values were determined using a nonparametric Mann–Whitney test. * indicates significant differences versus untreated mice (p < 0.05). Abbreviations used were NAM, NAM-treated mice using 1% NAM dissolved in tap water [85] (Julve et al., 2021, unpublished data).