Figure 3.

(A), Confocal fluorescent images of live and dead MRSA and P. aeruginosa cells after treatment with Cosolvent, the FAV and the FAH. Green fluorescence represents live bacteria stained with Calcein-AM, whereas red fluorescence represents dead bacteria stained with PI. (B,C,E), the corresponding quantitative assay of fluorescent intensity and the ATP level in MRSA and P. aeruginosa with different treatments. Inset data are ratios of live/dead bacteria. (D), SEM images of MRSA and P. aeruginosa treated with Cosolvent and the flavonoids extracts. Differences between groups with *** p < 0.001 was regarded as statistically significant.