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. 2022 Jan 5;11(1):173. doi: 10.3390/cells11010173

Figure 4.

Figure 4

Apoptosis is not the predominant form of programmed cell death that is rapidly triggered by the tested DS variants in the luminal breast cancer cell cultures. (A,C) The quantification of caspase-3/7-positive cells in the BT-483 (A) and T-47D (C) cultures that were grown exposed to NF and PM or DF and PM, respectively, for the indicated time periods. The number of the cells exhibiting caspase-3/7 activity was estimated in at least ten non-overlapping fields from each of two independent experiments. The results are expressed as a percentage of the apoptotic cells and are presented as the mean ± SEM for all of the obtained images. *—difference statistically significant (p ≤ 0.05) versus the control. (B,D) The representative images that show the number of caspase-3/7-positive cells in the BT-483 (B) or T-47D (D) cultures grown in the exposure to NF or DF, respectively for the time periods in which the maximal AnV binding had been observed. Caspase-positive cells are indicated by green fluorescence. The images were taken at a magnification of ×200.