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(a) Measured fluorescence of an interphase or M phase (mitotic) extract supplemented with securin-CFP as a function of time. An interphase extract supplemented with the blank control in vitro transcription/translation reaction was used as a background control. Shown are data from two technical replicates (full or empty circles respectively). (b) Data shown in (a) after background subtraction and normalization. The dashed or solid lines, respectively, show the exponential fit of the data
