Reagent or Resource	Source	Identifier
Chemicals, peptides, and recombinant proteins
2% glutaraldehyde in 0.1 M cacodylate buffer	N/A	N/A
3% potassium ferrocyanide	Sigma	Cat# P3289
0.1% thiocarbohydrazide	Electron Microscopy Sciences	Cat# 21900
2% osmium tetroxide	Electron Microscopy Sciences	Cat# 19112
1% uranyl acetate	Electron Microscopy Sciences	Cat# 22400-2
0.6% lead aspartate solution	MP Biomedicals	Cat# 155180
HPLC grade acetone	N/A	N/A
Epoxy 812 hard resin	Electron Microscopy Sciences	Cat# 14900
Experimental Models: Organisms/strains
Mouse: C57B16	Mayo Clinic	N/A
Drosophila: Mef2 Gal4	VDRC (Vienna) Drosophila stock center and Bloomington Drosophila stock center.	BS# 27390
Drosophila: W1118	VDRC (Vienna) Drosophila stock center and Bloomington Drosophila stock center.	N/A
Drosophila: Opa-1-like	VDRC (Vienna) Drosophila stock center and Bloomington Drosophila stock center.	N/A
Software and Algorithms
Amira Software	Thermo Scientific/Amira [61]	RRID: SCR_007353
Image J	Schneider et al. (2012) [62]	https://imagej.nih.gov/ij/ (assessed on 3 June 2021)
Ilastik	Berg et al. (2019) [63]	https://www.ilastik.org/publications.html (assessed on 3 June 2021)