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. 2021 Dec 24;14(1):78. doi: 10.3390/cancers14010078

Figure 4.

Figure 4

Activation of the unfolded protein response (UPR) triggers increased liver injury in Jnk∆hepa mice. (A) The expression of BiP/GRP78, CHOP, spliced XBP1 (sXBP1) and unspliced XBP1 (uXBP1) was evaluated in 32-week-old Jnkf/f and Jnk∆hepa mice using Western blot. (B) The expression of the same proteins was measured in Tunicamycin (TM)-challenged Jnkf/f and Jnk∆hepa mice. Numbers denote molecular weight (KDa) of proteins. GAPDH served as loading control. #/* p < 0.05 intra- and intergroup significance, respectively. (C) Representative Sirius Red (SR) stainings of liver tissue from the indicated mice after TM treatment. Scale bars, 500 μm. (D) TUNEL staining was performed to assess apoptotic cell death in the same samples by IF. Scale bars, 50 μm. (E) Ki67 was used to measure cell proliferation after TM treatment in the experimental groups. Scale bars, 50 μm. Data were represented as the mean ± SEM and graphed (n = 3–6 mice per group;*/# p < 0.05; ## p < 0.01; *** p < 0.001; ****/#### p < 0.0001).